Fig. 4

Efects of sodium butyrate on Tfh and B cell frequency and the representative cytokines and IgG antibodies levels. Mononuclear cells (MNCs) of the spleens were isolated from mice in three groups on day 70. A Tfh cells and C B cells were detected by fow cytometry. B The percentages of Tfh and D B cells in MNCs were calculated (n=3 mice/group). Levels of E IFN-γ, F IL-17 A, G IL-10, H IL-21, and I titer of IgG antibody in the blood were measured by ELISA (n=6 mice/group). Data were from three independent experiments and expressed as mean±SEM. The signifcance of diferences was assessed by ANOVA, followed by Tukey’s testing as a post-hoc test. ns means not signifcant, *p<0.05,**p<0.001