Fig. 3

Immunostaining of brain tissue shows a progressive accumulation of phosphorylated tau protein. a and b. Representative image of brain stem from P301S mice stained with (a) AT8 and (b) AT100 antibody shows i: No AT8 or AT100 tau signals were detected at brain of 2 months age P301S. ii and iii: show progressive accumulation of AT8 and AT100 reactive tau in P301S mice at 4 and 6 months respectively. c and d: Representative image from the hippocampus showing AT8 (c) and AT100 (d) reactive tau aggregates appears at 6 months P301S mice. Black arrows for tau apecefic signals, white arrow for nonspecific nuclear signal detected by AT100. (e) and (f): Bar graphs showing quantification of the AT8 and AT100 immunoreactive tau at the three age points represented as mean +/- SEM of brain sections from 4–6 mice per group. P value is tested by One-way ANOVA test followed with post hoc Tukey test where ** p < 0.01 and **** p < 0.0001. Scale bars = 100 μm