Fig. 2

Dio suppresses IL-1β-induced inflammatory cytokines expression in chondrocytes. Chondrocytes were exposed to IL-1β (10 ng/mL) with or without Dio (200, 400, and 800 ng/mL) for 24 h. A Griess reaction was used to determine the NO concentration. B PGE2 level was detected by ELISA. C Gene expressions of COX-2 and iNOS were detected by qRT-PCR. D Representative western blots and (E) quantitative analysis of COX-2 and iNOS in each group. ^#P < 0.05 vs. control group; ^*P < 0.05 vs. IL-1β group; ^**P < 0.01 vs. IL-1β group; n = 3