Fig. 6

CU06-1004 reduced NF-ĸB p65 activation in LPS-induced ALI. (A) Lung tissues from LPS-challenged mice showed NF-κB p65-positive staining using immunohistochemistry. Magnification, 200X (upper panel) and 400X (lower panel). Scale bar: 50 μm. (B) Expression of phosphor-NF-κB p65 and NF-κB p65 in mouse lung lysate was analyzed using western blotting; β-actin used as the loading control. (C) Quantitative graph of western blot analysis using ImageJ software as phospho-p65 per p65. N = 6 per group. Results presented as mean ± SEM values; ** p < 0.01, *** p < 0.001