Fig. 5
CircFADS2 directly targeted miR-133a. The putative binding sites between circFADS2 and miR-133a and circFADS2 MUT predicted by StarBase software (A). 1 × 106 HBEpCs were co-transfected with circFADS2-WT (or circFADS2-Mut) and miR-NC (or miR-133a). 48 h later, the luciferase activity was detected by Dual‑luciferase reporter assay (B). 1 × 106 HBEpCs were transfected with 1 µg CircFADS2 expression vector or 50nM miR-133a mimic. 48 h later, the expression levels of circFADS2 and miR-133a were detected by RT-qPCR(C). 1 × 106 HBEpCs were transfected with 1 µg CircFADS2 expression vector. 48 h later, miR-133a level was detected by RT-qPCR (D). *, p<0.05.